Interfacing digital microfluidics with high-field nuclear magnetic resonance spectroscopy.
نویسندگان
چکیده
Nuclear magnetic resonance (NMR) spectroscopy is extremely powerful for chemical analysis but it suffers from lower mass sensitivity compared to many other analytical detection methods. NMR microcoils have been developed in response to this limitation, but interfacing these coils with small sample volumes is a challenge. We introduce here the first digital microfluidic system capable of interfacing droplets of analyte with microcoils in a high-field NMR spectrometer. A finite element simulation was performed to assist in determining appropriate system parameters. After optimization, droplets inside the spectrometer could be controlled remotely, permitting the observation of processes such as xylose-borate complexation and glucose oxidase catalysis. We propose that the combination of DMF and NMR will be a useful new tool for a wide range of applications in chemical analysis.
منابع مشابه
High-resolution Nmr Spectroscopy on a Chip by Structural Compensation of Magnetic Susceptibility Mismatch
Microfluidic compensation structures are introduced as a tool to homogenize magnetic field gradients present in microfluidic NMR spectroscopy. Magnetic field distortions are eliminated by compensating the magnetic susceptibility mismatch between the sample fluid and chip material through additional, lithographically defined compensation structures. This strategy will allow high resolution NMR s...
متن کاملAnalytical detection techniques for droplet microfluidics--a review.
In the last decade, droplet-based microfluidics has undergone rapid progress in the fields of single-cell analysis, digital PCR, protein crystallization and high throughput screening. It has been proved to be a promising platform for performing chemical and biological experiments with ultra-small volumes (picoliter to nanoliter) and ultra-high throughput. The ability to analyze the content in d...
متن کاملA Multidisciplinary Approach to High Throughput Nuclear Magnetic Resonance Spectroscopy
Nuclear Magnetic Resonance (NMR) is a non-contact, powerful structure-elucidation technique for biochemical analysis. NMR spectroscopy is used extensively in a variety of life science applications including drug discovery. However, existing NMR technology is limited in that it cannot run a large number of experiments simultaneously in one unit. Recent advances in micro-fabrication technologies ...
متن کاملNMR-DMF: a modular nuclear magnetic resonance-digital microfluidics system for biological assays.
We present a modular nuclear magnetic resonance-digital microfluidics (NMR-DMF) system as a portable diagnostic platform for miniaturized biological assays. With increasing number of combinations between designed probes and a specific target, NMR has become an accurate and rapid assay tool, which is capable of detecting particular kinds of proteins, DNAs, bacteria and cells with a customized pr...
متن کاملروشهای طیفسنجی در آنالیز همزمان مواد منفجره
Simultaneous analysis of explosives to identify, determine the purity and eliminate environmental pollution requires rapid, simple and cheap methods. Spectrophotometries method (UV-Vis), high-field proton nuclear magnetic resonance (1H-NMR), Raman spectroscopy and near infrared (NIR) are common methods that are applicable in two ways of direct and indirect in simultaneous analysis. In this poin...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Lab on a chip
دوره 16 22 شماره
صفحات -
تاریخ انتشار 2016